– Plasmid Profile of Antibiotic Resistant Salmonella Species Isolated From Poultry Products –
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ABSTRACT
The increased prevalence of Salmonella contamination in poultry has gained considerable scientific attention during the last few decades.
The research work was done to determine the plasmid Species isolated from poultry products. In the reaserch, 24 strains of Salmonella isolated where tested to ten antibiotics using the disc diffusion method, to determine their susceptibility.
The zone of inhibition was observed and recorded in percentage. 24 strains showed 100% resistant to septrin, chloramphenicol, streptomycin, tarvid, agumentin, higher resistant was observed against amoxacilin (19%), Gentamicin (91%) and pefloxacin(91%).
The isolates showed lower resistant of 66% against sparfloxacin and ciprofloxacin. While 6 isolates was sensitivity against gentamicin, amoxacilin, sparfloxacin, and ciprofloxacin.
Six resistant strain of Salmonella isolates was selected at random to determine the plasmid profile. The result obtained showed that 1, 2, 6 in the DNA ladder are positive to plasmid with the molecular weight of 0.5kb – 48.5kb, while 3, 4, 5, are negative to plasmid.
INTRODUCTION
The emergence of has become a major public health concern. Salmonella species are widely distributed in the environment that causes a diverse spectrum of diseases in human and animals.
Non typhoidal Salmonella species are among the foremost implicated in food-borne gastroenteritis worldwide (Foley et al., 2006).
The World Health Organization (WHO) has estimated that annually 1.3 billion cases of acute gastroenteritis or diarrhea due to non typhoid salmonellosis causing 3 million deaths.
In India an estimated 4,00,000 children below 5 years age die each year due to diarrhoea (Sudershan et al., 2009).
Salmonella is most often transmitted to humans through the food chain, with over 95% of salmonellosis cases attributable to the consumption of undercooked or mishandled beef, chicken and eggs etc., (Foley et al., 2006).
A variety of have been used to treat the salmonellosis. An increasing rate of antimicrobial resistance in Salmonella has been reported in many developing and developed countries (Threlfall et al., 1993).
Briggs and Fratamsco (1999) reported that the frequency of resistance is presumably due to extensive use of antimicrobial agents in human and veterinary medicine.
Furthermore, resistance to combinations of several has led to the emergence of Multi-Drug Resistant (MDR) strains that may pass from food animals to humans (White et al., 2001).
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